Mouse BDNF ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

About this product

Mouse BDNF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse BDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).

Specifications

Catalog number

EZ0309-carrier-free

Size / count

5 Plates/Kit

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.

Synonyms

Brain-derived neurotrophic factor;BDNF;Bdnf;

Gene Name

Brain-derived neurotrophic factor

Gene ID

12064

UniProt

P21237

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: sf21; Immunogen sequence: H129-R247

Applications

ELISA

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Function

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.

Components

Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

28123 MW

Specificity

Natural and recombinant mouse BDNF

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted.

Protein Name

Brain-derived neurotrophic factor