Mouse TNF Alpha/Tumor necrosis factor ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

Catalog number EZ0527-carrier-free

Discontinued

Price for EZ0527-carrier-free

Size / count 5 Plates/Kit

This variant is discontinued and cannot be added to your cart.

About this product

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Tnf in cell culture supernatants, serum and plasma (heparin, EDTA).

Specifications

Catalog number: EZ0527-carrier-free
Size / count: 5 Plates/Kit
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
Gene Name: Tnf
Gene ID: 21926
UniProt: P06804
Reactivity: Mouse
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Immunogen: Expression system for standard: E.coli; Immunogen sequence: L80-L235
Applications: ELISA
Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range: 15.6 pg/ml - 1,000 pg/ml
Components: Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|
Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Specificity: Natural and recombinant mouse Tnf

Frequently Asked Questions

What is the catalog number for Mouse TNF Alpha/Tumor necrosis factor ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free?

The catalog number for Mouse TNF Alpha/Tumor necrosis factor ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free is EZ0527-carrier-free.

Is Mouse TNF Alpha/Tumor necrosis factor ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free in stock?

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Mouse TNF Alpha/Tumor necrosis factor ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free?

The safety data sheet for Mouse TNF Alpha/Tumor necrosis factor ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free is available here: https://www.bosterbio.com/msds?sku=EZ0527.

What is Mouse TNF Alpha/Tumor necrosis factor ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free used for?

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

Is this product classed as hazardous or restricted for shipping?

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Can you help me confirm if this product is right for my specific experiment?

Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.