Human TGFBR3/Tgf Beta Riii PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human TGFBR3/Tgf Beta Riii PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human TGFBR3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1383-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell- surfacechondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. Betaglycan binds to various members of the TGF-beta superfamily of ligands via its core protein, and bFGF via its heparan sulfate chains. It is not involved ly in TGF-beta signal transduction but by binding to various member of the TGF-beta superfamily at the cell surface it acts as a reservoir of ligand for TGF-beta receptors. By cDNA array and immunohistochemistry analyses, it is found that TGFBR3 expression is lost in most breast cancers examined in association with loss of heterozygosity of the TGFBR3 locus. TGFBR3 expression decreased during breast cancer progression, and low levels predicted decreased recurrence-free survival in patients. Loss of TGFBR3 through allelic imbalance is a frequent genetic event during breast cancer development that increases metastatic potential. Also, TGFBR3 can localize transformation in the heart and play an essential, nonredundant role in TGF-beta signaling.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>186</td><td>199</td><td>223</td><td>196</td><td>201</td><td>13.58</td><td>6.7%</td></tr><tr><td>Sample 2</td><td>1370</td><td>1157</td><td>1296</td><td>1222</td><td>1261</td><td>79.75</td><td>6.3%</td></tr><tr><td>Sample 3</td><td>5554</td><td>6224</td><td>5744</td><td>5545</td><td>5766</td><td>275.69</td><td>4.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Transforming growth factor beta receptor type 3;TGF-beta receptor type 3;TGFR-3;Betaglycan;Transforming growth factor beta receptor III;TGF-beta receptor type III;TGFBR3;

Gene Name

Transforming growth factor beta receptor type 3

Gene ID

7049

UniProt

Q03167

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G21-D781

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors.

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

93499 MW

Specificity

Natural and recombinant human TGFBR3

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted. Secreted, extracellular space. Cell membrane ; Single-pass type I membrane protein . Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix.

Protein Name

Transforming growth factor beta receptor type 3