Human RBP4/Retinol Binding Protein 4 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human RBP4/Retinol Binding Protein 4 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human RBP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0831-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c (Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>621</td><td>574</td><td>637</td><td>603</td><td>608</td><td>23.39</td><td>3.8%</td></tr><tr><td>Sample 2</td><td>2874</td><td>2703</td><td>2656</td><td>2896</td><td>2782</td><td>104.37</td><td>3.7%</td></tr><tr><td>Sample 3</td><td>9419</td><td>8739</td><td>8492</td><td>8429</td><td>8769</td><td>392.34</td><td>4.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Retinol-binding protein 4;Plasma retinol-binding protein;PRBP;RBP;Plasma retinol-binding protein (1-182);Plasma retinol-binding protein (1-181);Plasma retinol-binding protein (1-179);Plasma retinol-binding protein (1-176);RBP4;PRO2222;

Gene Name

Retinol-binding protein 4

Gene ID

5950

UniProt

P02753

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-L201

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Function

Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

23010 MW

Specificity

Natural and recombinant human RBP4

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the calycin superfamily. Lipocalin family.

Protein Name

Retinol-binding protein 4