Human Decorin / DCN PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human Decorin / DCN PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human DCN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0749-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Decorin is a proteoglycan on average 90 - 140 kilodaltons (kD) in size.It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>60</td><td>51</td><td>50</td><td>59</td><td>55</td><td>4.52</td><td>8.2%</td></tr><tr><td>Sample 2</td><td>247</td><td>279</td><td>302</td><td>287</td><td>278</td><td>20.1</td><td>7.2%</td></tr><tr><td>Sample 3</td><td>1001</td><td>962</td><td>922</td><td>976</td><td>965</td><td>28.61</td><td>2.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Decorin;Bone proteoglycan II;PG-S2;PG40;DCN;SLRR1B;

Gene Name

Decorin

Gene ID

1634

UniProt

P07585

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D31-K359

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

May affect the rate of fibrils formation.

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

39747 MW

Specificity

Natural and recombinant human Decorin

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Sequence Similarities

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Protein Name

Decorin