Human CD93/C1qR PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CD93/C1qR PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CD93 in cell culture supernatants, serum, plasma ( heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1320-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93 belongs to the Group XIV C-Type lectin family and is mapped to 20p11.21. The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. It has also showed that this receptor is a positive marker for all bone marrow-repopulating stem cells because it is expressed on both CD34-negative and CD34-positive stem cells from umbilical cord blood and adult bone marrow. In addition, highly purified lineage-negative cells with the CD93 protein not only have bone marrow-repopulating capacity but also can differentiate into human hepatocytes in vivo.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>94</td><td>96</td><td>95</td><td>92</td><td>94</td><td>1.47</td><td>1.5%</td></tr><tr><td>Sample 2</td><td>706</td><td>763</td><td>758</td><td>718</td><td>736</td><td>24.68</td><td>3.3%</td></tr><tr><td>Sample 3</td><td>2947</td><td>2642</td><td>2748</td><td>2847</td><td>2796</td><td>113.38</td><td>4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Complement component C1q receptor;C1q/MBL/SPA receptor;C1qR;C1qR (p);C1qRp;CDw93;Complement component 1 q subcomponent receptor 1;Matrix-remodeling-associated protein 4;CD93;CD93;C1QR1, MXRA4;

Gene Name

Complement component C1q receptor

Gene ID

22918

UniProt

Q9NPY3

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A24-K580

Tissue Specificity

Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma ( heparin, EDTA) , saliva and urine.

Detection Range

78 pg/ml - 5,000 pg/ml

Sensitivity

<10 pg/ml

Function

Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion.

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

68560 MW

Specificity

Natural and recombinant human CD93

Anticoagulant

heparin or EDTA

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 1 C-type lectin domain.

Protein Name

Complement component C1q receptor