Mouse SAP/PTX2/APCS PicoKine® Quick ELISA Kit, Carrier-free

About this product

Mouse SAP/PTX2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Apcs in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1208-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>3608</td><td>4012</td><td>4067</td><td>4138</td><td>3956</td><td>205.96</td><td>5.2%</td></tr><tr><td>Sample 2</td><td>12778</td><td>11342</td><td>12753</td><td>12065</td><td>12234</td><td>589.38</td><td>4.8%</td></tr><tr><td>Sample 3</td><td>47613</td><td>50001</td><td>47706</td><td>49348</td><td>48667</td><td>1034.13</td><td>2.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Serum amyloid P-component;SAP;Apcs;Ptx2, Sap;

Gene Name

Serum amyloid P-component

Gene ID

20219

UniProt

P12246

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-D224

Applications

ELISA

Standard

100 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

1.56 ng/ml - 100 ng/ml

Sensitivity

<50 pg/ml

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

26247 MW

Specificity

Natural and recombinant mouse SAP

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the pentaxin family.

Protein Name

Serum amyloid P-component