Human B7-1/CD80 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human B7-1/CD80 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CD80 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0707-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>162</td><td>138</td><td>164</td><td>139</td><td>150</td><td>12.27</td><td>8.1%</td></tr><tr><td>Sample 2</td><td>445</td><td>495</td><td>515</td><td>457</td><td>478</td><td>28.23</td><td>5.9%</td></tr><tr><td>Sample 3</td><td>1656</td><td>1667</td><td>1402</td><td>1560</td><td>1571</td><td>106.21</td><td>6.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

T-lymphocyte activation antigen CD80;Activation B7-1 antigen;BB1;CTLA-4 counter-receptor B7.1;B7;CD80;CD80;CD28LG, CD28LG1, LAB7;

Gene Name

T-lymphocyte activation antigen CD80

Gene ID

941

UniProt

P33681

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V35-N242

Tissue Specificity

Expressed on activated B-cells, macrophages and dendritic cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. .

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

33048 MW

Specificity

Natural and recombinant human CD80

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Protein Name

T-lymphocyte activation antigen CD80