Human Rantes PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human Rantes PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CCL5 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0494-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>35</td><td>35</td><td>39</td><td>38</td><td>36</td><td>1.78</td><td>4.9%</td></tr><tr><td>Sample 2</td><td>166</td><td>149</td><td>157</td><td>169</td><td>160</td><td>7.85</td><td>4.9%</td></tr><tr><td>Sample 3</td><td>442</td><td>377</td><td>390</td><td>418</td><td>406</td><td>25.17</td><td>6.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-C motif chemokine ;CCL5 ;hCG_32592 ;

Gene Name

C-C motif chemokine

Gene ID

6352

UniProt

D0EI67

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S24-S91

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<3 pg/ml

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

9990 MW

Specificity

Natural and recombinant human Rantes

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted .

Protein Name

C-C motif chemokine 5