Human CD25/IL-2sR Alpha PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CD25/IL-2sR Alpha PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human IL2RA in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0400-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>140</td><td>116</td><td>139</td><td>115</td><td>127</td><td>12.01</td><td>9.4%</td></tr><tr><td>Sample 2</td><td>719</td><td>735</td><td>616</td><td>709</td><td>694</td><td>46.4</td><td>6.6%</td></tr><tr><td>Sample 3</td><td>2353</td><td>2484</td><td>2499</td><td>2400</td><td>2434</td><td>60.08</td><td>2.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-2 receptor subunit alpha;IL-2 receptor subunit alpha;IL-2-RA;IL-2R subunit alpha;IL2-RA;TAC antigen;p55;CD25;IL2RA;

Gene Name

Interleukin-2 receptor subunit alpha

Gene ID

3559

UniProt

P01589

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-C213

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

78 pg/ml - 5,000 pg/ml

Sensitivity

<5 pg/ml

Function

Receptor for interleukin-2.

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

30819 MW

Specificity

Natural and recombinant human CD25

Anticoagulant

heparin or EDTA

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 2 Sushi (CCP/SCR) domains.

Protein Name

Interleukin-2 receptor subunit alpha