Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CEACAM5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0904-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>776</td><td>816</td><td>775</td><td>762</td><td>782</td><td>20.25</td><td>2.5%</td></tr><tr><td>Sample 2</td><td>1668</td><td>1752</td><td>1723</td><td>1646</td><td>1697</td><td>42.25</td><td>2.4%</td></tr><tr><td>Sample 3</td><td>10176</td><td>9323</td><td>9418</td><td>9597</td><td>9628</td><td>331.05</td><td>3.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Carcinoembryonic antigen-related cell adhesion molecule 5;Carcinoembryonic antigen;CEA;Meconium antigen 100;CD66e;CEACAM5;CEA;

Gene Name

Carcinoembryonic antigen-related cell adhesion molecule 5

Gene ID

111518

UniProt

P06731

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K35-A685

Tissue Specificity

Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Function

Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. .

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

76795 MW

Specificity

Natural and recombinant human CEA

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane ; Lipid-anchor, GPI-anchor .

Sequence Similarities

Belongs to the immunoglobulin superfamily. CEA family.

Protein Name

Carcinoembryonic antigen-related cell adhesion molecule 5