Human GDF-15 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human GDF-15 PicoKine&reg; <a href="https://www.bosterbio.com/elisa-kits/quick-elisa-kits-picokine-90-minutes" style="color:#EA8D28">Quick ELISA Kit</a> (120 minutes, 96 Tests). Quantitate Human GDF15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0767-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

GDF-15 (Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by ly interfering with chemokine signaling and integrin activation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>53</td><td>56</td><td>49</td><td>57</td><td>53</td><td>3.11</td><td>5.8%</td></tr><tr><td>Sample 2</td><td>252</td><td>245</td><td>249</td><td>271</td><td>254</td><td>9.98</td><td>3.9%</td></tr><tr><td>Sample 3</td><td>1068</td><td>1033</td><td>966</td><td>984</td><td>1012</td><td>40.23</td><td>3.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Growth/differentiation factor 15;GDF-15;Macrophage inhibitory cytokine 1;MIC-1;NSAID-activated gene 1 protein;NAG-1;NSAID-regulated gene 1 protein;NRG-1;Placental TGF-beta;Placental bone morphogenetic protein;Prostate differentiation factor;GDF15;MIC1, PDF, PLAB, PTGFB;

Gene Name

Growth/differentiation factor 15

Gene ID

9518

UniProt

Q99988

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: A197-I308

Tissue Specificity

Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

34140 MW

Specificity

Natural and recombinant human GDF-15

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .

Sequence Similarities

Belongs to the TGF-beta family.

Protein Name

Growth/differentiation factor 15