Human Laminin PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human Laminin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human Laminin in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0434-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>326</td><td>366</td><td>350</td><td>381</td><td>356</td><td>26.68</td><td>7.5%</td></tr><tr><td>Sample 2</td><td>1022</td><td>1228</td><td>1070</td><td>1230</td><td>1138</td><td>73.94</td><td>6.5%</td></tr><tr><td>Sample 3</td><td>4563</td><td>4223</td><td>4654</td><td>4640</td><td>4520</td><td>212.44</td><td>4.7%</td></tr></table>*number of samples for each test n=16.

Gene Name

Laminin subunit alpha-1

Gene ID

284217, 3908, 3909, 3910, 3911, 3912, 3913, 3914, 22798, 3915, 3918, 10319

UniProt

P25391, P24043, Q16787, Q16363, O15230, P07942, P55268, Q13751, A4D0S4, P11047, Q13753, Q9Y6N6

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Target protein isolated from human fibroblasts

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates and serum

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human Laminin

Protein Name

Laminin subunit alpha-1; Laminin subunit beta-1; Laminin subunit gamma-1