Rat EGF PicoKine® Quick ELISA Kit, Carrier-free

About this product

Rat EGF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Egf in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0954-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>12</td><td>11</td><td>10</td><td>11</td><td>11</td><td>0.36</td><td>3.3%</td></tr><tr><td>Sample 2</td><td>88</td><td>90</td><td>86</td><td>93</td><td>89</td><td>5.80</td><td>6.5%</td></tr><tr><td>Sample 3</td><td>241</td><td>212</td><td>229</td><td>243</td><td>231</td><td>8.79</td><td>3.8%</td></tr></table>*number of samples for each test n=16.

Gene Name

epidermal growth factor

Gene ID

25313

UniProt

P07522

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N974-R1026

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Detection Range

7.8 pg/ml - 500 pg/ml

Sensitivity

<1 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Rat Egf

Anticoagulant

heparin or EDTA

Protein Name

Pro-epidermal growth factor