Rat KIM1 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Rat KIM1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0882-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rat is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the rat Tim1 gene and database analysis, was mapped the HAVCR1 gene to 5q33.2.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>88</td><td>93</td><td>105</td><td>102</td><td>97</td><td>5.34</td><td>5.5%</td></tr><tr><td>Sample 2</td><td>289</td><td>271</td><td>296</td><td>302</td><td>290</td><td>16.79</td><td>5.8%</td></tr><tr><td>Sample 3</td><td>921</td><td>881</td><td>935</td><td>899</td><td>909</td><td>34.54</td><td>3.8%</td></tr></table>*number of samples for each test n=16.

Gene Name

hepatitis A virus cellular receptor 1

Gene ID

286934

UniProt

O54947

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S18-V238

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<2 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Rat Havcr1

Anticoagulant

heparin or EDTA

Protein Name

Hepatitis A virus cellular receptor 1