Rat Rantes PicoKine® Quick ELISA Kit, Carrier-free

About this product

Rat Rantes PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Ccl5 in cell culture supernatants. serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0496-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>32</td><td>30</td><td>36</td><td>29</td><td>32</td><td>1.65</td><td>5.2%</td></tr><tr><td>Sample 2</td><td>212</td><td>180</td><td>196</td><td>206</td><td>199</td><td>9.73</td><td>4.9%</td></tr><tr><td>Sample3</td><td>364</td><td>397</td><td>405</td><td>374</td><td>385</td><td>6.93</td><td>1.8%</td></tr></table>*number of samples for each test n=16.

Gene Name

chemokine (C-C motif) ligand 5

Gene ID

81780

UniProt

P50231

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S24-S91

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants. serum and plasma (heparin, EDTA, citrate)

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<1 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Rat Ccl5

Anticoagulant

heparin, EDTA or citrate

Protein Name

C-C motif chemokine 5