Human IL-1RA/IL1RN PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human IL-1RA/IL1RN PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human IL1RN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0782-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>55</td><td>57</td><td>61</td><td>51</td><td>56</td><td>2.52</td><td>4.5%</td></tr><tr><td>Sample 2</td><td>284</td><td>286</td><td>296</td><td>295</td><td>290</td><td>9.58</td><td>3.3%</td></tr><tr><td>Sample 3</td><td>853</td><td>884</td><td>831</td><td>924</td><td>873</td><td>49.76</td><td>5.7%</td></tr></table>*number of samples for each test n=16.

Gene Name

interleukin 1 receptor antagonist

Gene ID

3557

UniProt

P18510

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R26-E177

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<2 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human IL1RN

Anticoagulant

heparin or EDTA

Protein Name

Interleukin-1 receptor antagonist protein