Human ANGPTL3 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human ANGPTL3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ANGPTL3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1374-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>712</td><td>718</td><td>645</td><td>684</td><td>690</td><td>30.35</td><td>4.4%</td></tr><tr><td>Sample 2</td><td>1841</td><td>2134</td><td>2209</td><td>1998</td><td>2046</td><td>130.91</td><td>6.4%</td></tr><tr><td>Sample 3</td><td>9677</td><td>8512</td><td>10354</td><td>8424</td><td>9242</td><td>822.52</td><td>8.9%</td></tr></table>*number of samples for each test n=16.

Gene Name

angiopoietin-like 3

Gene ID

27329

UniProt

Q9Y5C1

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S17-E460

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human ANGPTL3

Anticoagulant

heparin or EDTA

Protein Name

Angiopoietin-related protein 3