Human Ficolin-1/FCN1/M Ficolin PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human Ficolin-1/FCN1/M Ficolin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human FCN1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1257-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1450</td><td>1365</td><td>1605</td><td>1564</td><td>1496</td><td>97.24</td><td>6.5%</td></tr><tr><td>Sample 2</td><td>8641</td><td>7641</td><td>7712</td><td>7952</td><td>7987</td><td>263.55</td><td>3.3%</td></tr><tr><td>Sample 3</td><td>19842</td><td>22647</td><td>21642</td><td>20458</td><td>21147</td><td>1141.95</td><td>5.4%</td></tr></table>*number of samples for each test n=16.

Gene Name

ficolin (collagen/fibrinogen domain containing) 1

Gene ID

2219

UniProt

O00602

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A30-A326

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin)

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<20 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human FCN1

Anticoagulant

heparin

Protein Name

Ficolin-1