Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ANGPTL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0960-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Angiopoietin-related protein 4 (Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1210</td><td>1128</td><td>1302</td><td>1147</td><td>1197</td><td>74.2</td><td>6.2%</td></tr><tr><td>Sample 2</td><td>4752</td><td>4862</td><td>5145</td><td>4510</td><td>4817</td><td>269.77</td><td>5.6%</td></tr><tr><td>Sample 3</td><td>21031</td><td>21450</td><td>20486</td><td>18843</td><td>20453</td><td>859.01</td><td>4.2%</td></tr></table>*number of samples for each test n=16.

Gene Name

angiopoietin-like 4

Gene ID

51129

UniProt

Q9BY76

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L165-S406

Applications

ELISA

Standard

40 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

625 pg/ml - 40,000 pg/ml

Sensitivity

<300 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human ANGPTL4

Anticoagulant

heparin or EDTA

Protein Name

Angiopoietin-related protein 4