Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human A2M in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1118-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1336</td><td>1254</td><td>1452</td><td>1396</td><td>1360</td><td>61.18</td><td>4.5%</td></tr><tr><td>Sample 2</td><td>3774</td><td>3674</td><td>3364</td><td>3354</td><td>3542</td><td>254.99</td><td>7.2%</td></tr><tr><td>Sample 3</td><td>26412</td><td>33012</td><td>29451</td><td>29456</td><td>29583</td><td>1804.55</td><td>6.1%</td></tr></table>*number of samples for each test n=16.

Gene Name

alpha-2-macroglobulin

Gene ID

2

UniProt

P01023

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Target protein isolated from human plasma

Applications

ELISA

Standard

40 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

625 pg/ml - 40,000 pg/ml

Sensitivity

<300 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human A2M

Anticoagulant

heparin or EDTA

Protein Name

Alpha-2-macroglobulin