Human CD56/NCAM-1 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CD56/NCAM-1 PicoKine® Quick ELISA Kit PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD56/NCAM-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0706-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

Reproducibility

<h2>Reproducibility</h2><p style="line-height: 0.4;"></p><p>To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.</p><table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>321</td><td>343</td><td>315</td><td>299</td><td>319</td><td>16.58</td><td>5.2%</td></tr><tr><td>Sample 2</td><td>1087</td><td>1191</td><td>1033</td><td>1021</td><td>1083</td><td>6.06</td><td>6.1%</td></tr><tr><td>Sample 3</td><td>5574</td><td>5246</td><td>5975</td><td>5765</td><td>5640</td><td>253.80</td><td>4.5%</td></tr></table>*number of samples for each test n=16.

Gene Name

NCAM1

Gene ID

4684

UniProt

P13591

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-G718

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human NCAM1

Anticoagulant

heparin or EDTA