Human APLP1 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human APLP1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human APLP1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK2103-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

This gene encodes a member of the highly conserved amyloid precursor protein gene family. It is mapped to 19q13.12. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described.

Reproducibility

<h2>Reproducibility</h2><p style="line-height: 0.4;"></p><p>To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.</p><table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>270</td><td>211</td><td>253</td><td>236</td><td>183</td><td>13.90</td><td>7.6%</td></tr><tr><td>Sample 2</td><td>972</td><td>1440</td><td>1371</td><td>1396</td><td>1294</td><td>54.34</td><td>4.2%</td></tr><tr><td>Sample 3</td><td>3395</td><td>4159</td><td>3479</td><td>3643</td><td>3669</td><td>194.45</td><td>5.3%</td></tr></table>*number of samples for each test n=16.

Gene Name

APLP1

Gene ID

333

UniProt

P51693

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S39-E580

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human APLP1

Anticoagulant

heparin or EDTA