Human CCL4/MIP-1 beta PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CCL4/MIP-1 beta PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL4/MIP-1 beta in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 4 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0450-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.

Reproducibility

<h2>Reproducibility</h2><p style="line-height: 0.4;"></p><p>To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.</p><table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>45</td><td>47</td><td>40</td><td>41</td><td>43</td><td>1.55</td><td>3.8%</td></tr><tr><td>Sample 2</td><td>333</td><td>237</td><td>173</td><td>219</td><td>240</td><td>18.48</td><td>7.7%</td></tr><tr><td>Sample 3</td><td>503</td><td>533</td><td>481</td><td>465</td><td>495</td><td>26.23</td><td>5.3%</td></tr></table>*number of samples for each test n=16.

Gene Name

CCL4

Gene ID

6351

UniProt

P13236

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-N92

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<4 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CCL4

Anticoagulant

heparin, EDTA or citrate