Human CD26/DPP4 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CD26/DPP4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD26/DPP4 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0696-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Dipeptidyl peptidase-4(DPP4), also known as adenosine deaminase complexing protein 2 or CD26(cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.

Reproducibility

<h2>Reproducibility</h2><p style="line-height: 0.4;"></p><p>To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.</p><table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>482</td><td>550</td><td>492</td><td>487</td><td>503</td><td>30.67</td><td>6.1%</td></tr><tr><td>Sample 2</td><td>2761</td><td>2826</td><td>2924</td><td>2841</td><td>2838</td><td>76.63</td><td>2.7%</td></tr><tr><td>Sample 3</td><td>8848</td><td>9902</td><td>8546</td><td>9412</td><td>9177</td><td>504.74</td><td>5.5%</td></tr></table>*number of samples for each test n=16.

Gene Name

DPP4

Gene ID

1803

UniProt

P27487

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D34-P766

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human DPP4

Anticoagulant

heparin or EDTA