Human Chemerin/RARRES2 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human Chemerin/RARRES2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human Chemerin/RARRES2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1329-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1(also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.

Reproducibility

<h2>Reproducibility</h2><p style="line-height: 0.4;"></p><p>To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.</p><table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>118</td><td>122</td><td>103</td><td>115</td><td>115</td><td>6.76</td><td>5.9%</td></tr><tr><td>Sample 2</td><td>754</td><td>766</td><td>687</td><td>729</td><td>734</td><td>27.16</td><td>3.7%</td></tr><tr><td>Sample 3</td><td>3266</td><td>2789</td><td>2969</td><td>3381</td><td>3101</td><td>192.28</td><td>6.2%</td></tr></table>*number of samples for each test n=16.

Gene Name

RARRES2

Gene ID

5919

UniProt

Q99969

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V17-S163

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

78 pg/ml - 5,000 pg/ml

Sensitivity

<20 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human RARRES2

Anticoagulant

heparin or EDTA