Human CFHR5 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CFHR5 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CFHR5 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 0.1 ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1933-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Complement factor H-related protein 5 is a protein that in humans is encoded by the CFHR5 gene. It is mapped to 1q31.3. This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS).

Reproducibility

<h2>Reproducibility</h2><p style="line-height: 0.4;"></p><p>To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.</p><table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1228</td><td>1345</td><td>1463</td><td>1512</td><td>1387</td><td>81.83</td><td>5.9%</td></tr><tr><td>Sample 2</td><td>5795</td><td>6654</td><td>5963</td><td>5794</td><td>6052</td><td>387.3</td><td>6.4%</td></tr><tr><td>Sample 3</td><td>26491</td><td>29746</td><td>25641</td><td>26758</td><td>27272</td><td>1472.66</td><td>5.4%</td></tr></table>*number of samples for each test n=16.

Gene Name

CFHR5

Gene ID

81494

UniProt

Q9BXR6

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-E569

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA) and urine

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<0.1 ng/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CFHR5

Anticoagulant

heparin or EDTA