Human CCL13/MCP4 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CCL13/MCP4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL13/MCP4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0809-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Chemokine(C-C motif) ligand 13(CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.

Reproducibility

<h2>Reproducibility</h2><p style="line-height: 0.4;"></p><p>To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.</p><table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>19</td><td>18</td><td>17</td><td>17</td><td>18</td><td>1.26</td><td>7.1%</td></tr><tr><td>Sample 2</td><td>87</td><td>85</td><td>88</td><td>82</td><td>86</td><td>2.05</td><td>2.4%</td></tr><tr><td>Sample 3</td><td>258</td><td>234</td><td>248</td><td>251</td><td>248</td><td>11.15</td><td>4.5%</td></tr></table>*number of samples for each test n=16.

Gene Name

CCL13

Gene ID

6357

UniProt

Q99616

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-T98

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

7.8 pg/ml - 500 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CCL13

Anticoagulant

heparin or EDTA