Mouse TNFRSF7/CD27 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Mouse TNFRSF7/CD27 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse TNFRSF7/CD27 in cell culture supernatants and serum. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0699-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein(SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Reproducibility

<h2>Reproducibility</h2><p style="line-height: 0.4;"></p><p>To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.</p><table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>84</td><td>85</td><td>81</td><td>93</td><td>86</td><td>3.77</td><td>4.4%</td></tr><tr><td>Sample 2</td><td>579</td><td>605</td><td>594</td><td>616</td><td>599</td><td>31.72</td><td>5.3%</td></tr><tr><td>Sample 3</td><td>1861</td><td>1745</td><td>2051</td><td>2133</td><td>1948</td><td>138.27</td><td>7.1%</td></tr></table>*number of samples for each test n=16.

Gene Name

Cd27

Gene ID

21940

UniProt

P41272

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T21-R182

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Cd27

Anticoagulant

/