Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit, Carrier-free

About this product

Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1313-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Reproducibility

<h2>Reproducibility</h2><p style="line-height: 0.4;"></p><p>To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.</p><table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>88</td><td>87</td><td>94</td><td>85</td><td>89</td><td>4.69</td><td>5.3%</td></tr><tr><td>Sample 2</td><td>208</td><td>235</td><td>216</td><td>244</td><td>226</td><td>11.06</td><td>4.9%</td></tr><tr><td>Sample 3</td><td>904</td><td>867</td><td>905</td><td>841</td><td>879</td><td>39.57</td><td>4.5%</td></tr></table>*number of samples for each test n=16.

Gene Name

Fcgr2b/Fcgr3

Gene ID

14130/14131

UniProt

P08101/P08508

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T40-R217

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Fcgr2b/Fcgr3

Anticoagulant

heparin or EDTA