Human CD40/TNFRSF5 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CD40/TNFRSF5 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD40/TNFRSF5 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0702-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

Reproducibility

<h2>Reproducibility</h2><p style="line-height: 0.4;"></p><p>To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.</p><table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>33</td><td>31</td><td>34</td><td>35</td><td>33</td><td>2.19</td><td>6.6%</td></tr><tr><td>Sample 2</td><td>165</td><td>170</td><td>178</td><td>180</td><td>173</td><td>7.8</td><td>4.5%</td></tr><tr><td>Sample 3</td><td>415</td><td>425</td><td>433</td><td>501</td><td>444</td><td>11.53</td><td>2.6%</td></tr></table>*number of samples for each test n=16.

Gene Name

CD40

Gene ID

958

UniProt

P25942

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-R193

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<1 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CD40

Anticoagulant

heparin or EDTA