Human CCL14/HCC-1 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CCL14/HCC-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1123-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Chemokine(C-C motif) ligand 14(CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.

Reproducibility

<h2>Reproducibility</h2><p style="line-height: 0.4;"></p><p>To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.</p><table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>42</td><td>39</td><td>41</td><td>40</td><td>40</td><td>1.36</td><td>3.4%</td></tr><tr><td>Sample 2</td><td>232</td><td>255</td><td>256</td><td>221</td><td>241</td><td>13.49</td><td>5.6%</td></tr><tr><td>Sample 3</td><td>450</td><td>506</td><td>528</td><td>504</td><td>497</td><td>25.84</td><td>5.2%</td></tr></table>*number of samples for each test n=16.

Gene Name

CCL14

Gene ID

6358

UniProt

Q16627

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T20-N93

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant human CCL14

Anticoagulant

heparin or EDTA