Human BMP-4 ELISA Kit PicoKine®, Carrier-free

About this product

Human BMP-4 ELISA Kit PicoKine® (96 Tests). Quantitate Human BMP4 in cell culture supernatants, cell lysates and serum. Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0314-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>196</td><td>183</td><td>176</td><td>194</td><td>187</td><td>8.16</td><td>4.3%</td></tr><tr><td>Sample 2</td><td>814</td><td>793</td><td>763</td><td>851</td><td>805</td><td>32.03</td><td>3.9%</td></tr><tr><td>Sample 3</td><td>1756</td><td>1763</td><td>1749</td><td>1644</td><td>1728</td><td>48.74</td><td>2.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Bone morphogenetic protein 4;BMP-4;Bone morphogenetic protein 2B;BMP-2B;BMP4;BMP2B, DVR4;

Gene Name

Bone morphogenetic protein 4

Gene ID

652

UniProt

P12644

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S293-R408

Tissue Specificity

Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates and serum.

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<4 pg/ml

Function

Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity). .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

46555 MW

Specificity

Natural and recombinant human BMP-4

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Sequence Similarities

Belongs to the TGF-beta family.

Protein Name

Bone morphogenetic protein 4