Human FAS/Cd95 ELISA Kit PicoKine®, Carrier-free

About this product

Human FAS/Cd95 ELISA Kit PicoKine® (96 Tests). Quantitate Human FAS in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 3 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0335-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo. The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>68</td><td>60</td><td>71</td><td>60</td><td>64</td><td>4.86</td><td>7.5%</td></tr><tr><td>Sample 2</td><td>235</td><td>248</td><td>233</td><td>262</td><td>244</td><td>11.62</td><td>4.7%</td></tr><tr><td>Sample 3</td><td>915</td><td>855</td><td>754</td><td>803</td><td>831</td><td>59.88</td><td>7.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Tumor necrosis factor receptor superfamily member 6;Apo-1 antigen;Apoptosis-mediating surface antigen FAS;FASLG receptor;CD95;FAS;APT1, FAS1, TNFRSF6;

Gene Name

Tumor necrosis factor receptor superfamily member 6

Gene ID

355

UniProt

P25445

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R17-N173

Tissue Specificity

Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<3 pg/ml

Function

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

37732 MW

Specificity

Natural and recombinant human sFAS

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 1 death domain.

Protein Name

Tumor necrosis factor receptor superfamily member 6