Mouse G-CSF / CSF3 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse G-CSF / CSF3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Csf3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0361-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17.3 The standard product used in this kit is recombinant Mouse G-csf, consisting of 179 amino acids with the molecular mass of 19KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>54</td><td>53</td><td>52</td><td>59</td><td>54</td><td>2.69</td><td>4.9%</td></tr><tr><td>Sample 2</td><td>247</td><td>297</td><td>262</td><td>252</td><td>264</td><td>19.52</td><td>7.3%</td></tr><tr><td>Sample 3</td><td>1009</td><td>966</td><td>904</td><td>972</td><td>962</td><td>37.7</td><td>3.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Granulocyte colony-stimulating factor;G-CSF;Csf3;Csfg;

Gene Name

Granulocyte colony-stimulating factor

Gene ID

12985

UniProt

P09920

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V31-A208

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<4 pg/ml

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

22421 MW

Specificity

Natural and recombinant mouse G-CSF

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Protein Name

Granulocyte colony-stimulating factor