Mouse IGF-1 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse IGF-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Igf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0378-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD.1 The IGF1 gene, mapped on 12q22-q24. contains 5 exons. Exons 1-4 encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>75</td><td>79</td><td>75</td><td>90</td><td>79</td><td>6.13</td><td>7.7%</td></tr><tr><td>Sample 2</td><td>604</td><td>579</td><td>666</td><td>572</td><td>605</td><td>37.03</td><td>6.1%</td></tr><tr><td>Sample 3</td><td>1350</td><td>1408</td><td>1325</td><td>1461</td><td>1386</td><td>52.73</td><td>3.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Insulin-like growth factor I ;Igf1 ;

Gene Name

insulin-like growth factor 1 (somatomedin C)

Gene ID

16000

UniProt

E9PU89

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with IGF-2.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G49-A118

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<5 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

15992 MW

Specificity

Natural and recombinant mouse IGF-1

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .

Sequence Similarities

Belongs to the insulin family.

Protein Name

Insulin-like growth factor I