Mouse IL-5/Interleukin-5 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse IL-5/Interleukin-5 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il5 in cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0408-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>36</td><td>31</td><td>37</td><td>35</td><td>34</td><td>2.27</td><td>6.6%</td></tr><tr><td>Sample 2</td><td>220</td><td>228</td><td>267</td><td>256</td><td>242</td><td>19.35</td><td>7.9%</td></tr><tr><td>Sample 3</td><td>453</td><td>402</td><td>397</td><td>434</td><td>421</td><td>23.07</td><td>5.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-5;IL-5;B-cell growth factor II;BCGF-II;Cytotoxic T-lymphocyte inducer;Eosinophil differentiation factor;T-cell replacing factor;TRF;Il5;Il-5;

Gene Name

Interleukin-5

Gene ID

16191

UniProt

P04401

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M21-G133

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<2 pg/ml

Function

Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

15410 MW

Specificity

Natural and recombinant mouse IL-5

Subcellular Location

Secreted.

Protein Name

Interleukin-5