Rat IL-6/Interleukin-6 ELISA Kit PicoKine®, Carrier-free

About this product

Rat IL-6/Interleukin-6 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Il6 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0412-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6 (IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone (CRH). Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor (IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>193</td><td>192</td><td>175</td><td>177</td><td>184</td><td>8.28</td><td>4.5%</td></tr><tr><td>Sample 2</td><td>579</td><td>631</td><td>610</td><td>577</td><td>599</td><td>22.52</td><td>3.7%</td></tr><tr><td>Sample 3</td><td>1531</td><td>1470</td><td>1340</td><td>1471</td><td>1453</td><td>69.76</td><td>4.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-6;IL-6;Il6;Il-6;

Gene Name

Interleukin-6

Gene ID

24498

UniProt

P20607

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F25-T211

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<5 pg/ml

Function

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T (H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity). .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

24357 MW

Specificity

Natural and recombinant rat IL-6

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted.

Protein Name

Interleukin-6