Rat Laminin ELISA Kit PicoKine®, Carrier-free

About this product

Rat Laminin ELISA Kit PicoKine® (96 Tests). Quantitate Rat Laminin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0435-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>256</td><td>282</td><td>284</td><td>258</td><td>270</td><td>13.03</td><td>4.8%</td></tr><tr><td>Sample 2</td><td>1435</td><td>1315</td><td>1498</td><td>1568</td><td>1454</td><td>93.02</td><td>6.3%</td></tr><tr><td>Sample 3</td><td>4770</td><td>4867</td><td>4216</td><td>4900</td><td>4688</td><td>276.8</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Laminin subunit beta-2;Laminin chain B3;Laminin-11 subunit beta;Laminin-14 subunit beta;Laminin-15 subunit beta;Laminin-3 subunit beta;Laminin-4 subunit beta;Laminin-7 subunit beta;Laminin-9 subunit beta;S-laminin subunit beta;S-LAM beta;Lamb2;

Gene Name

Laminin subunit beta-2

UniProt

D4A409, F1M614, D3ZN05, F1LTF8, F1MAN8, D3ZQN7, P15800, F1LPI5, F1MAA7, F1LRH4, A0A0G2K023

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Target protein isolated from rat sarcoma basement membrane

Tissue Specificity

Found in the basement membranes (major component). S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

196474 MW

Specificity

Natural rat Laminin

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

Sequence Similarities

Contains 13 laminin EGF-like domains.

Protein Name

Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2