Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse MMP-3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Mmp3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0462-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>281</td><td>283</td><td>271</td><td>287</td><td>280</td><td>5.89</td><td>2.1%</td></tr><tr><td>Sample 2</td><td>1427</td><td>1569</td><td>1617</td><td>1589</td><td>1550</td><td>73.31</td><td>4.7%</td></tr><tr><td>Sample 3</td><td>4432</td><td>4951</td><td>4788</td><td>4684</td><td>4713</td><td>188.46</td><td>3.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Stromelysin-1;SL-1;3.4.24.17;EMS-2;Matrix metalloproteinase-3;MMP-3;Transin-1;Mmp3;

Gene Name

Stromelysin-1

Gene ID

17392

UniProt

P28862

Reactivity

Mouse

Cross Reactivity

There is cross-reactivity with MMP-10 approximately 2% and no detectable cross-reactivity with other MMPs.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y18-C477

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

53845 MW

Specificity

Natural and recombinant mouse total MMP-3

Anticoagulant

heparin

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sequence Similarities

Belongs to the peptidase M10A family.

Protein Name

Stromelysin-1