Human MMP-7/Matrilysin ELISA Kit PicoKine®, Carrier-free

About this product

Human MMP-7 ELISA Kit PicoKine® (96 Tests). Quantitate Human MMP7 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 6pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0463-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Matrix metalloproteinase-7 (MMP-7) previously called putative metalloproteinase I (PUMP1) or matrilysin. The PUMP1 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The PUMP I protein has 267 amino acids and is significantly shorter than stromelysin or collagenase (477 and 469 amino acids, respectively). Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. Matrilysin, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, matrilysin mRNA is regulated in a specific and distinct manner in normal and malignant tissues. Matrilysin has been shown to correlate with nodal or distant metastasis in colorectal carcinomas; however, its implication in early invasive colorectal carcinomas has not been determined.1 Matrilysin is also a mediator of pulmonary fibrosis and a potential therapeutic target. The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28KDa. The detected MMP-7 includes zymogen and active enzyme.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>272</td><td>280</td><td>250</td><td>279</td><td>270</td><td>12.09</td><td>4.4%</td></tr><tr><td>Sample 2</td><td>1042</td><td>1051</td><td>1050</td><td>1108</td><td>1062</td><td>26.35</td><td>2.4%</td></tr><tr><td>Sample 3</td><td>5090</td><td>5484</td><td>5287</td><td>4635</td><td>5124</td><td>314.81</td><td>6.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Matrilysin;3.4.24.23;Matrin;Matrix metalloproteinase-7;MMP-7;Pump-1 protease;Uterine metalloproteinase;MMP7;MPSL1, PUMP1;

Gene Name

Matrilysin

Gene ID

4316

UniProt

P09237

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L18-K267

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin) , saliva and urine.

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<6 pg/ml

Function

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

29677 MW

Specificity

Natural and recombinant human total MMP-7

Anticoagulant

heparin

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sequence Similarities

Belongs to the peptidase M10A family.

Protein Name

Matrilysin