Human MMP-9 ELISA Kit PicoKine®, Carrier-free

About this product

Human MMP-9 ELISA Kit PicoKine® (96 Tests). Quantitate Human MMP9 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0465-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>237</td><td>203</td><td>233</td><td>214</td><td>221</td><td>13.88</td><td>6.2%</td></tr><tr><td>Sample 2</td><td>1530</td><td>1745</td><td>1683</td><td>1555</td><td>1628</td><td>88.94</td><td>5.4%</td></tr><tr><td>Sample 3</td><td>5547</td><td>5421</td><td>5245</td><td>5758</td><td>5492</td><td>186.96</td><td>3.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Matrix metalloproteinase-9;MMP-9;3.4.24.35;92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB;67 kDa matrix metalloproteinase-9;82 kDa matrix metalloproteinase-9;MMP9;CLG4B;

Gene Name

Matrix metalloproteinase-9

Gene ID

4318

UniProt

P14780

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-D707

Tissue Specificity

Produced by normal alveolar macrophages and granulocytes.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin) , saliva and urine.

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<5 pg/ml

Function

May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

78458 MW

Specificity

Natural and recombinant human total MMP-9

Anticoagulant

heparin

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sequence Similarities

Belongs to the peptidase M10A family.

Protein Name

Matrix metalloproteinase-9