Rat NGF/NGF Beta ELISA Kit PicoKine®, Carrier-free

About this product

Rat NGF/NGF Beta ELISA Kit PicoKine® (96 Tests). Quantitate Rat Ngf in cell culture supernatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0471-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>33</td><td>32</td><td>33</td><td>35</td><td>33</td><td>1.08</td><td>3.2%</td></tr><tr><td>Sample 2</td><td>191</td><td>172</td><td>185</td><td>192</td><td>185</td><td>7.96</td><td>4.3%</td></tr><tr><td>Sample 3</td><td>480</td><td>451</td><td>440</td><td>436</td><td>451</td><td>17.21</td><td>3.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Beta-nerve growth factor;Beta-NGF;Ngf;Ngfb;

Gene Name

Beta-nerve growth factor

Gene ID

310738

UniProt

P25427

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S122-G241

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<1 pg/ml

Function

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

27010 MW

Specificity

Natural and recombinant rat NGF

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the NGF-beta family.

Protein Name

Beta-nerve growth factor