Human OPN / Osteopontin ELISA Kit PicoKine®, Carrier-free

About this product

Human OPN / Osteopontin ELISA Kit PicoKine® (96 Tests). Quantitate Human SPP1 in cell culture supernatants, serum, plasma (heparin, EDTA) , human milk and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0482-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>305</td><td>309</td><td>272</td><td>322</td><td>302</td><td>18.42</td><td>6%</td></tr><tr><td>Sample 2</td><td>1771</td><td>1751</td><td>1709</td><td>1739</td><td>1742</td><td>22.46</td><td>1.2%</td></tr><tr><td>Sample 3</td><td>4985</td><td>4178</td><td>4476</td><td>4405</td><td>4511</td><td>294.96</td><td>6.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Osteopontin;Bone sialoprotein 1;Nephropontin;Secreted phosphoprotein 1;SPP-1;Urinary stone protein;Uropontin;SPP1;BNSP, OPN;PSEC0156;

Gene Name

Osteopontin

Gene ID

6696

UniProt

P10451

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I17-N300

Tissue Specificity

Bone. Found in plasma.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) , human milk and urine.

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

35423 MW

Specificity

Natural and recombinant human OPN

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the osteopontin family.

Protein Name

Osteopontin