- Catalog number
- EK0513-carrier-free
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- Yes
- Background
- Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative ion of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.
- Reproducibility
- <table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>30</td><td>29</td><td>32</td><td>28</td><td>29</td><td>1.47</td><td>5%</td></tr><tr><td>Sample 2</td><td>156</td><td>166</td><td>150</td><td>158</td><td>157</td><td>5.72</td><td>3.6%</td></tr><tr><td>Sample 3</td><td>497</td><td>448</td><td>471</td><td>476</td><td>473</td><td>17.42</td><td>3.6%</td></tr></table>*number of samples for each test n=16.
- Synonyms
- Transforming growth factor beta-1;TGF-beta-1;Latency-associated peptide;LAP;TGFB1;TGFB;
- Gene Name
- Transforming growth factor beta-1
- Gene ID
- 7040
- UniProt
- P01137
- Reactivity
- Human
- Cross Reactivity
- There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: A279-S390
- Tissue Specificity
- Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage. .
- Applications
- ELISA
- Standard
- 10 ng/tube
- Sample Type
- cell culture supernatants, serum, plasma (EDTA) and urine.
- Detection Range
- 15.6 pg/ml - 1,000 pg/ml
- Sensitivity
- <1 pg/ml
- Function
- Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T- helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. .
- Components
- Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4
- Storage Conditions
- Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 44341 MW
- Specificity
- Natural and recombinant human TGF beta1
- Anticoagulant
- EDTA
- Subcellular Location
- Secreted, extracellular space, extracellular matrix .
- Sequence Similarities
- Belongs to the TGF-beta family.
- Protein Name
- Transforming growth factor beta-1
