Human E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine®, Carrier-free

About this product

Human E-Cadherin 1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CDH1 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0561-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

E-Cadherin, also called Cadherin 1 (CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca (2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>265</td><td>266</td><td>300</td><td>267</td><td>274</td><td>14.73</td><td>5.3%</td></tr><tr><td>Sample 2</td><td>1264</td><td>1074</td><td>1156</td><td>1112</td><td>1151</td><td>71.13</td><td>6.1%</td></tr><tr><td>Sample 3</td><td>4789</td><td>4996</td><td>5212</td><td>5707</td><td>5176</td><td>341.11</td><td>6.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Cadherin-1;CAM 120/80;Epithelial cadherin;E-cadherin;Uvomorulin;CD324;E-Cad/CTF1;E-Cad/CTF2;E-Cad/CTF3;CDH1;CDHE, UVO;

Gene Name

Cadherin-1

Gene ID

999

UniProt

P12830

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D155-I707

Tissue Specificity

Non-neural epithelial tissues.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin) , saliva and urine.

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<15 pg/ml

Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

97456 MW

Specificity

Natural and recombinant human E-Cadherin

Anticoagulant

heparin

Subcellular Location

Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

Sequence Similarities

Contains 5 cadherin domains.

Protein Name

Cadherin-1