Human TNF Beta/Lymphotoxin-alpha ELISA Kit PicoKine®, Carrier-free

About this product

Human TNF Beta ELISA Kit PicoKine® (96 Tests). Quantitate Human LTA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0584-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin-alpha (LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35% identity and 50% homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>165</td><td>162</td><td>154</td><td>142</td><td>155</td><td>8.89</td><td>5.7%</td></tr><tr><td>Sample 2</td><td>487</td><td>454</td><td>458</td><td>413</td><td>453</td><td>26.37</td><td>5.8%</td></tr><tr><td>Sample 3</td><td>1791</td><td>1778</td><td>1760</td><td>1730</td><td>1764</td><td>22.88</td><td>1.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Lymphotoxin-alpha;LT-alpha;TNF-beta;Tumor necrosis factor ligand superfamily member 1;LTA;TNFB, TNFSF1;

Gene Name

Lymphotoxin-alpha

Gene ID

4049

UniProt

P01374

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L35-L205

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<5 pg/ml

Function

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

22297 MW

Specificity

Natural and recombinant human TNF beta

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.

Sequence Similarities

Belongs to the tumor necrosis factor family.

Protein Name

Lymphotoxin-alpha