Mouse Prolactin ELISA Kit PicoKine®, Carrier-free

About this product

Mouse Prolactin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Prl in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0594-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>247</td><td>250</td><td>249</td><td>260</td><td>251</td><td>5.02</td><td>2%</td></tr><tr><td>Sample 2</td><td>1548</td><td>1485</td><td>1544</td><td>1578</td><td>1538</td><td>33.69</td><td>2.1%</td></tr><tr><td>Sample 3</td><td>4124</td><td>4396</td><td>4363</td><td>4724</td><td>4401</td><td>213.61</td><td>4.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Prolactin;PRL;Prl;

Gene Name

Prolactin

Gene ID

19109

UniProt

P06879

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L32-C228

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, citrate).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Prolactin acts primarily on the mammary gland by promoting lactation.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

25496 MW

Specificity

Natural and recombinant mouse Prolactin

Anticoagulant

heparin or citrate

Subcellular Location

Secreted.

Protein Name

Prolactin