- Catalog number
- EK0659-carrier-free
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- Yes
- Background
- Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene, which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants.
- Reproducibility
- <table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>103</td><td>88</td><td>85</td><td>85</td><td>90</td><td>7.46</td><td>8.2%</td></tr><tr><td>Sample 2</td><td>593</td><td>706</td><td>654</td><td>601</td><td>638</td><td>45.47</td><td>7.1%</td></tr><tr><td>Sample 3</td><td>1295</td><td>1325</td><td>1154</td><td>1246</td><td>1255</td><td>64.77</td><td>5.1%</td></tr></table>*number of samples for each test n=16.
- Synonyms
- Tyrosine-protein kinase receptor UFO;2.7.10.1;AXL oncogene;AXL;UFO;
- Gene Name
- Tyrosine-protein kinase receptor UFO
- Gene ID
- 558
- UniProt
- P30530
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E33-P440
- Tissue Specificity
- Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue. .
- Applications
- ELISA
- Standard
- 10 ng/tube
- Sample Type
- cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
- Detection Range
- 62.5 pg/ml - 4,000 pg/ml
- Sensitivity
- <10 pg/ml
- Function
- Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3- kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor. .
- Components
- Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4
- Storage Conditions
- Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 98336 MW
- Specificity
- Natural and recombinant human AXL
- Anticoagulant
- heparin or EDTA
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein .
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
- Protein Name
- Tyrosine-protein kinase receptor UFO
