Human P-Cadherin-3 CDH3 ELISA Kit PicoKine®, Carrier-free

About this product

Human P-Cadherin-3 CDH3 ELISA Kit PicoKine® (96 Tests). Quantitate Human CDH3 in cell culture supernatants and serum. Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0667-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma. The standard product used in this kit is recombinant P-Cadherin with the molecular mass of 120-130Kda after glycosylation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>262</td><td>177</td><td>211</td><td>243</td><td>223</td><td>7.28</td><td>3.3%</td></tr><tr><td>Sample 2</td><td>1066</td><td>781</td><td>868</td><td>1158</td><td>968</td><td>63.71</td><td>6.6%</td></tr><tr><td>Sample 3</td><td>3298</td><td>3822</td><td>3030</td><td>2921</td><td>3268</td><td>184.95</td><td>5.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Cadherin-3;Placental cadherin;P-cadherin;CDH3;CDHP;

Gene Name

Cadherin-3

Gene ID

1001

UniProt

P22223

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D108-G654

Tissue Specificity

Expressed in some normal epithelial tissues and in some carcinoma cell lines. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<20 pg/ml

Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

91418 MW

Specificity

Natural and recombinant human P-Cadherin

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 5 cadherin domains.

Protein Name

Cadherin-3